CONTENTS:- Introduction; DRA, RNA and protein synthesis; Recombinant DNA technology; Chemical Synthesis; Sequencing and Amplification of DNA; Gene Function in Eukaryotes; Directed Mutagenesis and protein Engineering; Vaccines; Antibiotics and proteins made by Bacteria; Bioremediation; Plant genetic engineering; Methodology; Plant genetic Engineering; Applications; Transgenic Animals; Human Molecular Genetics; Human gene therapy; Regulatory and ethical aspects; biotechnology inventions; Gel Electrophoresis; cDNA Libraries; Genomic libraries; protein purification; Assays, specific Activity and initial fractionation; Protein purification; Molecular imprinting.
Written for students, this book covers both the underlying scientific principles and the industrial, agricultural pharmaceutical and biomedical applications of recombinant DNA technology. The discovery that DNA was the key to life was the spark that lit the Biotechnology revolution of the 20th century. DNA was the key, which allowed scientists to manipulate life on the molecular level. If the cells do not produce the correct proteins, don't worry change its DNA. Can't wait long enough for nature to produce the correct strain? Change the DNA. From uncertain beginnings, molecular biotechnology has now established itself as the centerpiece of Biotechnology. In fact, much of the populations still equate genetic engineering and biotechnology. We are now on the verge of a brave new future. Over the past two decades, biologists have acquired a very powerful tool in the form of molecular biotechnology. These techniques have led the analysis of biological systems in detail undreamed of only a generation ago. This book fills the need for a well-organised and logical presentation of the principles and applications of biotechnology.