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Plant Tissue Culture: Theory and Practice / Sinha, Satish Kumar (Dr.)
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Plant Tissue Culture: Theory and Practice
Sinha, Satish Kumar (Dr.)
 
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List Price : US$ 89.71
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  Book ID : 38916
  ISBN-10 : 93-5030-079-6 / 9350300796
  ISBN-13 : 978-93-5030-079-4 / 9789350300794
  Place of Publication : Jaipur
  Year of Publication : 2012
  Edition : (First Edition)
  Language : English
  viii, 272p., Bib., Index
   
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 CONTENTS
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CONTENTS:-
1. Introduction.
2. Applications of cell and tissue culture.
3. Applications of cell culture.
4. Methods of sterilization and disinfestations.
5. Micropropagation in plants.
6. Somatic embryogenesis principles, concepts and applications.
7. Indexing for plant pathogens.
8. Plant tissue techniques for crop improvement.
9. Plant breeding: induced mutation technology for crop improvement.
10. Embryo rescue and embryo culture.
11. Secondary metabolite production.
12. Agrobacterium mediated transformation.
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 DESCRIPTION
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The issue obtained from the plant to culture is called an explant. Based on work with certain model systems, particularly tobacco, it has often been claimed that a totipotent explant can be grown from any part of the plant. However, this concept has been vitiated in practice. In many species explants of various organs vary in their rates of growth and regeneration, while some do not grow at all. The choice of explant material also determines if the plantlets developed via tissue culture are haploid or diploid. Also the risk of microbial contamination is increased with inappropriate explants. Thus it is very important that an appropriate choice of explant be made prior to tissue culture. The specific differences in the regeneration potential of different organs and explants have various explanations. The significant factors include differences in the stage of the cells in the cell cycle, the availability of or ability to transport endogenous growth regulators, and the metabolic capabilities of the cells. The most commonly used tissue explants are the meristematic ends of the plants like the stem tip, auxiliary bud tip and root tip. These tissues have high rates of cell division and either concentrate or produce required growth regulating substances including auxins and cytokinins.

This book has been intended primarily for the students in undergraduate and postgraduate courses in agriculture. It is hoped that he publication is useful not only to the students but also to the academic professionals.

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